Figure 2.

The effect of R-(-)-β-O-methylsynephrine on retinal angiogenesis in mice with oxygen-induced retinopathy. The compound was intravitreously injected once into the mice (10 mice per group) on P14, and the inhibitory effect of the compound was examined at P17. (A) Retinal vasculatures in control and R-(-)-β-O-methylsynephrine (OMe-Syn; 20 μM)-treated mice with oxygen-induced retinopathy (OIR) were evaluated with fluorescein angiography. Neovascular tufts of intravitreous region were observed at the border of vascular and avascular retina. Scale bar: 500 μm. (B, C) Vascular lumens were counted to quantify retinal neovascularization in OIR mice. Cross-sections were prepared from postnatal day 17 control and OMe-Syn (20 μM)-treated mice for hematoxylin and eosin staining. Arrows indicate the vascular lumens of new vessels growing into the vitreous region. The number of vascular lumens was counted in randomly selected images under 200× magnification. Scale bar: 100 μm; ^**p<0.01 versus control (Student's t-test).