Figure 4.

The effect of R-(-)-β-O-methylsynephrine on the association of RNA with the RNA-binding domain of nucleoporin 153 kDa. (A) A competitive electrophoretic mobility shift assay was performed using a radiolabeled B38 RNA and the purified recombinant RNA-binding domain (RBD) of nucleoporin 153 kDa (NUP153). Various concentrations of R-(-)-β-O-methylsynephrine (OMe-Syn) and its inactive analog synephrine were tested for the competition of binding between B38 RNA and the NUP153-RBD. The representative image from three independent experiments is shown. (B) The relative intensity of the signals from the RNA-protein complexes was analyzed by phosphorimager. Data represent mean ± standard error from three independent experiments; ^**p<0.01 versus no compound control (One sample t-test).