Table 3. Physical and functional interactions between the proteins of the thiamin and thiazolium proteomes of rat brain synaptosomes.
| Protein name | Interaction partner | Proteome |
References | Type of interaction | |
|---|---|---|---|---|---|
| T | Tz | ||||
| Malate dehydrogenase (mitochondrial) | Glutamate dehydrogenase | − | + | 94 | Protein-protein interactions |
| Citrate synthase | − | + | 95 | Protein-protein interactions | |
| Aspartate aminotransferase (mitochondrial) | + | + | 96 | Protein-protein interactions | |
| Glutamate dehydrogenase | Malate dehydrogenase (mitochondrial) | − | + | 94 | Protein-protein interactions |
| Aspartate aminotransferase (mitochondrial) | − | + | 94 | Protein-protein interactions | |
| Pyridoxal kinase | Aspartate aminotransferase | + | + | 97 | Protein-protein interactions |
| 14-3-3 | Pyridoxal kinase | + | + | 98,99 | Protein-protein interactions |
| Profilin | − | + | 100 | Protein-protein interactions | |
| Actin | + | + | 100 | Protein-protein interactions | |
| Tubulin | − | + | 100 | Protein-protein interactions | |
| PP1-alfa | − | + | 98 | Protein-protein interactions | |
| Casein kinase II | + | − | 98 | Protein-protein interactions | |
| Peroxiredoxin 2 | − | + | 98 | Protein-protein interactions | |
| Peroxiredoxin 5 | − | + | 98 | Protein-protein interactions | |
| Peroxiredoxin 6 | − | + | 98 | Protein-protein interactions | |
| HSP70 | + | + | 98 | Protein-protein interactions | |
| Glutamine synthase | + | − | 84,101 | Protein-protein interactions Functional interaction: concomitant oxidation of 14-3-3 and glutamine synthase in Amyloid beta neurotoxicity | |
| Calmodulin | V-type ATPase, subunit A | − | + | 102 | Protein-protein interactions |
| BASP1 | − | + | 103 | Protein-protein interactions | |
| MARCKS | − | + | 104 | Protein-protein interactions | |
| Synapsin | − | + | 105 | Protein-protein interactions | |
| V-type ATPase (subunits A, B, D, E1) | Aldolase A, C | + | − | 106 | Protein-protein interactions |
| BASP1 | MARCKS | − | + | 107 | Protein-protein interactions |
| Actin | − | + | 108 | Protein-protein interactions | |
| MARCKS | HSP70 | − | + | 109 | Protein-protein interactions |
| Actin | − | + | 108 | Protein-protein interactions | |
| Synapsin | Rab3A | − | + | 110 | Functional interaction; Rab3A inhibited synapsin I binding to F-actin, as well as synapsin-induced actin bundling and vesicle clustering |
| Spectrin | − | + | 111 | Protein-protein interactions | |
| F-actin | − | + | 112 | Protein-protein interaction in a phosphorylation-dependent manner | |
| Peroxiredoxin 6 | Glutation-S-transferase Pi (GST Pi) | − | + | 113 | Functional interaction: oxidation of the catalytic cysteine in Prdx6 is required for its interaction with GST Pi |
| Amyloid β A4 protein | − | + | 114 | Functional interaction: Prdx 6 protects PC12 cells from Aβ25-35-induced neurotoxicity | |
| Amyloid β A4 protein | Serum Albumin | − | + | 115 | Protein-protein interactions |
| Protein phosphatase 2A (PP2A) | − | + | 116 | Protein-protein interactions (with amyloid β) | |
| Glutamate receptor 2 (GRIA2) | Tubulin | − | + | 117 | Protein-protein interactions |
| WW domain-binding protein 2 (WWP2) | + | − | 118 | Functional interaction: ADAR2 protein levels are regulated by WWP2 and this may have downstream effects on GRIA2 | |
| Casein kinase II | + | − | 119 | Functional interaction: casein kinase II phosphorylates GRIA2 | |
| Ubiquitin-dependent proteins | Ubiquitin carboxyl-terminal hydrolase 1 | − | + | 120 | Proteins and targets of ubiquitin system |
| Actin, cytoplasmic | + | + | |||
| Tubulin, alpha chain | + | + | |||
| Heat shock cognate 71 kDa protein | + | + | |||
| Dihydropyrimidinase-related protein 2 | + | + | |||
| 14-3-3 protein zeta/delta | + | + | |||
| Guanine nucleotide-binding protein G(o) subunit alpha | − | + | |||
| Synapsin-2 | − | + | |||
| Amyloid β A4 protein | − | + | |||
| Endophilin | Synaptojanin | − | + | 121 | Protein-protein interactions |
| PP1-alpha | − | + | 122 | Protein-protein interactions | |
| Aldose reductase | Hydroxyacylglutathione hydrolase mitochondrial (glyoxalase II) | + | − | 123 | Aldose reductase, glyoxalase I,and glyoxalase II are involved in the metabolism of methylglyoxal |
| Tubulin | − | + | 124 | Protein-protein interactions | |
HSP70, Heat shock 70 kDa protein; BASP1, Brain acid soluble protein 1; MARCKS, Myristoylated alanine-rich C-kinase substrate.