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. 2015 Jul 27;5:12595. doi: 10.1038/srep12595

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(A) Shows HPRT gene alone, (B) ratio 1:1, (C) ratio 1:2 and (D) ratio 1:10. We extracted both denaturation (green) and extension (red) curves from all graphs. No template control (NTC) expressed no amplification and its average fluorescence amplitude was 0.113 with a standard deviation of 0.002.