Table 4. Experiments with excess HPRT and extracted threshold cycles (C_T).

Experiment Name	Concentration HPRT in ng/μl	Concentration GAPDH in ng/μl	Denaturation CT	Extension CT
1:1	6.25 × 10−7	6.25 × 10−7	29.0	18.2
2:1	12.5 × 10−7	6.25 × 10−7	26.4	18.2
10:1	6.25 × 10−6	6.25 × 10−7	24.0	17.0
20:1	12.5 × 10−6	6.25 × 10−7	22.6	16.3
100:1	6.25 × 10−5	6.25 × 10−7	20.4	13.9