Table 5. Experiments with excess HPRT and extracted threshold cycles (C_T).

Experiment Name	Concentration HPRT in ng/μl	Concentration HA in ng/μl	Denaturation CT	Extension CT
1:1	6.25 × 10−7	6.25 × 10−7	28.6	19.2
2:1	12.5 × 10−7	6.25 × 10−7	26.3	18.3
10:1	6.25 × 10−6	6.25 × 10−7	23.3	16.6
20:1	12.5 × 10−6	6.25 × 10−7	22.7	15.9
100:1	6.25 × 10−5	6.25 × 10−7	19.6	14.1