Table 1.
Effect on antioxidant parameters in absence (CdCl2) and presence of AEIA (AEIA + CdCl2) and AEEF (AEEF + CdCl2) in isolated mice hepatocytes
| Parameters | Control | CdCl2 (30 µM) | AEIA (400 µg/ml) + CdCl2 (30 µM) | AEEF (400 µg/ml) + CdCl2 (30 µM) |
|---|---|---|---|---|
| TBARS (µg/g of tissue) | 3.65 ± 0.24 | 6.89 ± 0.54# | 4.89 ± 0.45* | 5.11 ± 0.51* |
| Protein carbonyl (nmol/mg of protein) | 41.75 ± 4.24 | 76.54 ± 5.33# | 52.82 ± 4.79** | 54.11 ± 3.96** |
| CAT (U/mg of protein) | 234.56 ± 14.33 | 168.67 ± 10.89# | 234.56 ± 14.92** | 228.92 ± 12.78* |
| SOD (U/mg of protein) | 86.54 ± 4.12 | 55.54 ± 3.96# | 75.44 ± 4.06** | 72.12 ± 4.35* |
| GST (µmol/h/mg protein) | 0.92 ± 0.11 | 0.54 ± 0.07$ | 0.88 ± 0.09* | 0.83 ± 0.07 |
| GPx (nmol/min/mg of protein) | 76.67 ± 4.12 | 51.78 ± 3.50# | 71.58 ± 4.21** | 67.43 ± 3.98** |
| GR (nmol/min/mg of protein) | 82.01 ± 4.45 | 54.15 ± 3.92# | 72.67 ± 4.05* | 70.84 ± 4.96* |
| G6PD (nmol/min/mg of protein) | 116.12 ± 5.56 | 75.60 ± 4.85# | 104.78 ± 5.11** | 101.96 ± 4.76** |
| GSH (nmol/mg of protein) | 5.23 ± 0.47 | 3.12 ± 0.34# | 4.81 ± 0.40* | 4.67 ± 0.33* |
Values are expressed as mean ± SE (n = 3). #Values differ significantly from normal control (p < 0.01). $ Values differ significantly from normal control (p < 0.05). * Values differ significantly from CdCl2 control (p < 0.05). ** Values differ significantly from CdCl2 control (p < 0.01). CAT unit, ‘U’ is defined as µmoles of H2O2 consumed per minute. SOD unit, ‘U’ is defined as the µmoles inhibition of NBT reduction per minute.