Table 1.
Parameter | Endpoint reading mode | Kinetic reading mode (AUC) |
---|---|---|
| ||
Linearity | 1) Y= −1400 ln x + 8932.2 R2=0.9936 |
1) Y= −657.3 ln x + 3883.0 R2=0.9906 |
2) Y= −1512 ln x + 9152.2 R2=0.9957 |
2) Y= −639 ln x + 3812.6 R2=0.9991 |
|
3) Y= −1183 ln x + 8503.3 R2=0.9913 |
3) Y= −923.5 ln x + 5066.1 R2=0.9869 |
|
| ||
IC 50 | 11.07 μg/mL ± 0.95 | 11.95 μg/mL ± 1.28 |
| ||
Linear range | 62.5 - 0.78 μg/mL | 62.5 - 0.78 μg/mL |
| ||
LOD / LOQ | LOD: 1.14 μg/mL | LOD: 2.45 μg/mL |
LOQ: 3.47 μg/mL | LOQ: 7.43 μg/mL |