Table 1.
Example modifications from the 523 ΔM bins.
| Bin Numbera | Average Δmass (Da) | Counts | Predicted modificationb | Localized Amino Acidc |
|---|---|---|---|---|
|
| ||||
| 305 | +79.967 | 1866 | Phosphorylation |
|
| 317 | +125.900 | 362 | Iodine |
|
| 333 | +183.037 | 2290 | Aminoethylbenzenesulfonylation |
|
| 264 | +27.995 | 3285 | Formylation |
|
| 299 | +72.005 | 88 | Unknown N-terminal (peptide) tryptophan |
|
| 265 | +28.032 | 244 | Dimethylation |
|
| 281 | +46.042 | 152 | Unknown cysteine modification (or 103.063 Da mod not considering alkylation) |
|
| 224 | −9.031 | 232 | Oxidation to cysteic acid |
|
| 180 | −113.087 | 437 | In-source dissociation of N-terminal Leucine or Isoleucine |
|
| 189 | −89.031 | 1934 | Cleaved N-terminal methionine + Acetylation |
|
| 170 | −131.042 | 673 | Cleaved N-terminal methionine |
|
| 276 | +42.018 | 956 | N-terminal Acetylation |
|
a
Guassian peak bin number associated with Supplementary tables 2 and 3.
b
Predicted modification based on the high resolution Δmass and known modifications.
c
Potential amino acid location.