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Autoradiogram showing induction of TG synthesis from 14C-labeled precursors by NO treatment in M. tuberculosis. After 4 h of NO treatment, cells were incubated with [1-14C]palmitic acid (for 1 h) and [1-14C]acetate (for 4 h), and the lipids were separated by TLC using n-hexane-ethyl ether-formic acid (45:5:1 [vol/vol/vol]) as the solvent system. S, spermine control; N, NO treatment. The bar graph shows the percentage of total administered radioactivity incorporation into TG. O, origin.
