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. Author manuscript; available in PMC: 2015 Jul 27.
Published in final edited form as: Autoimmunity. 2014 May 1;47(5):341–350. doi: 10.3109/08916934.2014.910768

Figure 2.

Figure 2

ELISPOT assay measuring antigen-specific IL-10, IFN-γ and IL-2 producing splenocytes of treated animals at 11 weeks (open bar) and 15 weeks (closed bar) upon re-stimulation with IBC and B9-23 ex vivo. (A and B) IL-10 producing cells stimulated with B:9-23 or IBC peptides. (C and D) IFN-γ-producing cells stimulated with B:9-23 or IBC peptides. (E and F) IL-2-producing cells stimulated with B:9-23 or IBC peptides. Two or three mice per group were analyzed at each time point. Parameters were tested in triplicate, with 5 × 105 splenocytes/well. Error bars indicate the range of the duplicate or triplicate measurements.