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. Author manuscript; available in PMC: 2015 Jul 27.
Published in final edited form as: J Infect Dis. 2007 Dec 15;196(12):1761–1772. doi: 10.1086/522966

Figure 5.

Figure 5

Expression of T cell receptor (TCR)–Vβ transcripts in enriched DRB1*0101/Env380-394 tetramer+ cells and in whole peripheral blood mononuclear cells (PBMCs) from patients with human T lymphotropic virus type 1–associated myelopathy/tropical spastic paraparesis (HAM/TSP). Expression of TCR-Vβ transcripts in enriched DRB1*0101/Env380-394 (DR1/Env) tetramer+CD4+ T cells and whole PBMCs from 2 patients with HAM/TSP (HAM1 and HAM2) were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR). Two microliters of the final TCR-Vβ RT-PCR product mixture were subjected to electrophoresis on a 5% polyacrylamide sequencing gel, and the resulting bands were detected on an automated sequencer. The relative amounts of Vβ transcripts in enriched DR1/Env tetramer+CD4+ T cells (gray bars) and total PBMCs (black bars) were quantified by fluorescence with GeneScan software.