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. Author manuscript; available in PMC: 2015 Jul 27.
Published in final edited form as: Cancer Clin Oncol. 2012 Nov;1(2):49–80. doi: 10.5539/cco.v1n2p49

Figure 2.

Figure 2

Biological and Physical Properties. Left Panel 1: Detection of total immunoglobulin bound to the exterior surface membrane of mammary adenocarcinoma in the form of covalent epirubicin immunochemotherapeutic. Legend: (■) covalent epirubicin-(C3-amide)-[anti-HER2/neu] immunochemotherapeutic; and (■) covalent epirubicin-(C3-amide)-SS-[anti-HER2/neu] immunochemotherapeutic. Mammary adenocarcinoma (SKBr-3) monolayer populations were incubated with the covalent epirubicin-(C3-amide)-[anti-HER2/neu] immunochemotherapeutics over a 4-hour period and total immunoglobulin bound on the exterior surface membrane was measured by cell-ELISA analysis. Right Panel 2: Western-blot chemiluminescent autoradiography of covalent epirubicin-(C3-amide)-[anti-HER2/neu] and epirubicin-(C3-amide)-SS-[anti-HER2/neu] immunochemotherapeutics. Legend: (Lane-1) murine anti-human HER2/neu immunoglobulin; (Lane-2) covalent epirubicin-(C3-amide)-[anti-HER2/neu] immunochemotherapeutic; and (Lane-3) covalent epirubicin-(C3-amide)-SS-[anti-HER2/neu] immunochemotherapeutic. Immunoglobulin preparations were mass-separated by SDS-PAGE and transferred laterally onto sheets of nitrocellulose membrane to facilitate detection with biotinylated goat anti-mouse IgG. Subsequent analysis entailed incubation of nitrocellulose membranes with conjugated strepavidin-HRPO in combination with the use of an HRPO chemiluminescent substrate to facilitate the acquisition of autoradiography images.