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. Author manuscript; available in PMC: 2015 Jul 27.
Published in final edited form as: Int J Parasitol. 2014 Apr 12;44(7):485–496. doi: 10.1016/j.ijpara.2014.03.005

Fig. 1.

Fig. 1

Cloning strategy for reporter and rescuing constructs. The constructs containing the Caenorhabditis elegans Ce-daf-2 promoter (pL-CG2) and the Haemonchus contortus Hc-daf-2 promoter (pL-HG2) were made based on pPD95.75 by overlap extension PCR. Rescuing constructs containing coding sequences of Ce-daf-2 (pL-CD2) and Hc-daf-2 (pL-HD2) were made by removing the gfp coding sequence from pPV238 (Massey et al., 2013) and linking the appropriate cDNA. A, B and P represent the restriction sites for AgeI, BstZ17I and PstI, respectively. UTR, untranslated region.