Table 4.
Effect of PKA pathway modulators on the uptake of [3H]Folic acid in Y-79 cells
| Modulators (µM) | Uptake (fmol/(min mg) protein) |
|---|---|
| Control | 23.11 ± 3.19 |
| IBMX | |
| 0.1 | 21.44 ± 1.30 |
| 1 | 18.09 ± 0.97* |
| 2.5 | 15.07 ± 2.48* |
| 5 | 10.83 ± 1.01* |
| IBMX (2.5 µM) + H-89 (100 µM) | 25.18 ± 1.91 |
| Forskolin | |
| 1 | 24.08 ± 3.11 |
| 10 | 19.71 ± 2.09 |
| 50 | 13.29 ± 0.97* |
| 100 | 10.01 ± 2.17* |
| Forskolin (50 µM) + H-89 (100 µM) | 22.17 ± 3.15 |
In these studies cells were preincubated with 1 ml of PKA pathway modulators (IBMX, H-89 and Forskolin) and the uptake of [3H]Folic acid was measured. Values are means ± S.E. of five separate uptake determinations.
Significant difference from control (p < 0.05).