Table 5.
Effect of PKC pathway modulators on the uptake of [3H]Folic acid in Y-79 cells
| Modulators (µM) | Uptake (fmol/(min mg) protein) |
|---|---|
| Control | 25.17 ± 2.16 |
| PMA | |
| 10 | 20.01 ± 1.21* |
| 50 | 14.29 ± 2.64* |
| 100 | 10.72 ± 1.43* |
| PMA (100 µM) + bisindolylmaleimide I | |
| 10 | 27.03 ± 3.11 |
| 50 | 21.92 ± 2.92 |
| 100 | 23.02 ± 1.56 |
In these studies cells were preincubated with 1 ml of PKC pathway activator (phorbol-12-myristate-13-acetate) and PKC pathway inhibitor (bisindolylmaleimide I) and the uptake of [3H]Folic acid was measured. Values are means ± S.E. of five separate uptake determinations.
Significant difference from control (p < 0.05).