TABLE 6.
Distribution of genomic islands of Y. pestis in Y. pseudotuberculosis
| Island designation in this studya | Range in CO92 chromosome | Annotated functions | Presence in Y. pseudotuberculosisb | Gene(s) selected for PCR-based screeningc |
|---|---|---|---|---|
| 01 | YPO0255-0273 | Type III secretion system | Yes | |
| 02 | YPO0335-0340 | Insect viral enhancin homologue | Yes | |
| 03 | YPO0590-0642 | Adhesin, autotransporter, secreted proteins, and protein kinase | Yes | |
| 04 | YPO0684-0697 | Adherence proteins | Yes | |
| 05 | YPO0770-0778 | Siderophore biosynthesis | Yes | |
| 06 | YPO0803-0818 | Type II secretion system | Yes | |
| 07 | YPO0871-0887 | Bacteriocin and prophage | Yes | |
| 08 | YPO0961-0995 | Quorum sensing system and siderophore biosynthesis | Yes | |
| 09 | YPO1083-1098 | Prophage | No | YPO1091 |
| 10 | YPO1224-1259 | Outer membrane protease and prophage | Yes | |
| 11 | YPO1448-1480 | Cytotoxic nectotizing factor homologue | Yes | |
| 12 | YPO1900-1917 | Yersiniabactin biosynthesis (HPI) | Yes | |
| 13 | YPO1951-1954 | Hemin storage system (Hms) | Yes | |
| 14-1 | YPO2087-2095 | Prophage | No | YPO2089 |
| 14-2 (DFR 12) | YPO2096-2135 | Prophage | No | YPO2098 and YPO2115 |
| 15 (DFR 13) | YPO2271-2280 | Prophage | No | YPO2273 |
| 16 | YPO2311-2321 | Insecticidal toxin | Yes | |
| 17 | YPO2434-2443 | Iron transport and antibiotic resistance | Yes | |
| 18 | YPO2863-2868 | Membrane proteins | Yes | |
| 19 | YPO2934-2948 | Chaperone/usher fimbrial system | Yes | |
| 20 | YPO3673-3682 | Insecticidal toxin | Yes | |
| 21 | YPO4014-4033 | Iron transport | Yes |
a
Island 14 was divided into two subislands (island 14-1 and island 14-2) because island 14-1 is present in all of the Y. pestis strains tested but absent from Y. pseudotuberculosis strains, whereas island 14-2 (DFR 12) was found to be absent from both Microtus strains and Y. pseudotuberculosis strains.
b
Based on microarray analysis of 36 isolates of Y. pestis and 7 strains of Y. pseudotuberculosis.
c
These genes were selected as target genes in PCR analysis to represent the relevant islands; PCR screening of 260 isolates of Y. pestis and 7 strains of Y. pseudotuberculosis confirmed the microarray results.