Fig 3. PspA induces PD-L1 expression in a MyD88 dependent pathway.

Mouse bone marrow derived DCs were transfected with siRNAs against indicated molecules for 36h followed by stimulations with 1 μg/ml Pam₃Csk₄ and 15 μg/ml PspA for 24h. PD-L1 levels were monitored by flow cytometry. Dotted line represents unstimulated cells transfected with control siRNAs. Thin lines represent cells transfected with control siRNAs followed by stimulations with 1 μg/ml Pam₃Csk₄ and 15 μg/ml PspA. Bold lines represent cells transfected with specific siRNAs to indicated molecules followed by stimulations with 1 μg/ml Pam₃Csk₄ and 15 μg/ml PspA. Data from one of three independent experiments are shown. In Panel B, PD-L1 expression is represented as bars indicating fold increase in Relative Mean Fluorescence Intensity (MFI) for various groups. Bars represent mean ± S.D. of three independent experiments.