TABLE 1.
Purification of PPC from M. thermautotrophicus
| Purification step | Volume (ml) | Protein (mg) | Sp act (U/mg) | Total act (U) | Purification (fold) | % Recovery |
|---|---|---|---|---|---|---|
| Ultracentrifugation | 112 | 4,200 | 0.015 | 63 | ||
| Ammonium sulfate precipitation | 100 | 43.5 | 0.391a | 17a | 26 | 27 |
| DEAE-Sephadex chromatography | 32.5 | 5.68 | 5.5a | 31.3a | 367 | 50 |
| Concentration | 20 | 4.8 | 7.37 | 35.4 | 492 | 56 |
| Hydroxypatite chromatography | 10.5 | 1.05 | 23.2 | 24.3 | 1,543 | 39 |
| Octyl Sepharose chromatography | 14 | 0.07 | 260 | 18.2 | 17,333 | 29 |
a
The reported value is an underestimate, because ammonium sulfate and sodium chloride inhibited the enzyme.