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. Author manuscript; available in PMC: 2016 Jun 9.
Published in final edited form as: Biochemistry. 2015 May 28;54(22):3514–3527. doi: 10.1021/acs.biochem.5b00286

Table 4.

Equilibrium dissociation constants for CheY binding to FliM1–16 peptide.

CheY designation Amino acid at position Kd FliM1–16(μM)a
D+2 T+2
DX set (HAD mimics)
 DR D R 65 ± 5
 DK D K 120 ± 8
 DY D Y 370 ± 30
 DQ D Q 380 ± 50
NX set
 NR N R 180 ± 9
 NK N K 230 ± 40
 NY N Y 160 ± 10
wild type N E 370 ± 20
a

Mean and standard deviation, n=3.