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Degradation of 3MGA and gallate by insertion mutants. One millimolar 3MGA (A) and gallate (B) was incubated with the cell extracts (20 and 5 mg of protein for 3MGA and gallate degradation, respectively) of SYK-6 (solid diamonds), DB (open triangles), DZ (open squares), and DBZ (open circles). The degradation activities of the cell extracts toward these substrates were determined by GC-MS analysis. Each value is the average ± standard deviation of three independent experiments.
