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Tetrahydrofolate-dependent transformation of 3MGA to gallate. 3MGA (1 mM) was incubated with the cell extract of DBZ (10 mg of protein) in the presence of 1 mM tetrahydrofolate. (A and B) Gas chromatograms of TMS derivatives of the reaction products at the start and after 3 h of incubation, respectively. (C) Mass spectrum of the compound with a retention time at 30.2 min in panel B.
