TABLE 1.
B. subtilis strains used
| B. subtilis strain | Genotypea | Source or reference |
|---|---|---|
| 168 | trpC2 | Laboratory stock |
| BSIP1214 | trpC2 ytlI::aphA3 | 7 |
| BSIP1256 | trpC2 amyE::(PytmI′-lacZ cat) | pDIA5599 → 168 |
| BSIP1321 | trpC2 amyE::(PyhcL′-lacZ cat) | pDIA5629 → 168 |
| BSIP1389 | trpC2 ΔytmJKLMN::aphA3 | —b |
| BSIP1398 | trpC2 ΔytmJKLMN::aphA3 yhcL′::lacZ-erm ΔyhcL | BSIP1389 → BFS1605 |
| BSIP1534 | trpC2 ΔyhcL::spc | pDIA5669 → 168 |
| BSIP1570 | trpC2 ΔyxeMNO::cat | — |
| BSIP1572 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat | BSIP1389 → BSIP1570 |
| BSIP1575 | trpC2 ΔyxeMNO::cat ΔyhcL::spc | BSIP1570 → BSIP1534 |
| BSIP1576 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat ΔyhcL::spc | BSIP1534 → BSIP1572 |
| BSIP1582 | trpC2 ΔytmJKLMN::aphA3 ΔyhcL::spc | BSIP1389 → BSIP1534 |
| BSIP1601 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat ΔyhcL::lacZ-erm ΔyhcL | BSIP1570 → BSIP1398 |
| BSIP1603 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat yhcL′::lacZ-erm ΔyhcL yckK::Tn10 | Transposon mutagenesis |
| BSIP1643 | trpC2 ΔytmJKLMN::aphA3 yckK′::lacZ-erm ΔyckK | BSIP1389 → BFS4376 |
| BSIP1644 | trpC2 ΔyxeMNO::cat yckK′::lacZ-erm ΔyckK | BSIP1570 → BFS4376 |
| BSIP1645 | trpC2 ΔyhcL::spc yckK′::lacZ-erm ΔyckK | BSIP1534 → BFS4376 |
| BSIP1646 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat yckK′::lacZ-erm ΔyckK | BFS4376 → BSIP1572 |
| BSIP1647 | trpC2 ΔyxeMNO::cat ΔyhcL::spc yckK′::lacZ-erm ΔyckK | BFS4376 → BSIP1575 |
| BSIP1648 | trpC2 ΔytmJKLMN::aphA3 ΔyxeMNO::cat ΔyhcL::spc yckK′::lacZ-erm ΔyckK | BFS4376 → BSIP1576 |
| BSIP1649 | trpC2 ΔytmJKLMN::aphA3 ΔyhcL::spc yckK′::lacZ-erm ΔyckK | BFS4376 → BSIP1582 |
| BSIP1689 | trpC2 amyE::(PyhcL′-lacZ cat) ΔytmJKLMN::aphA3 | BSIP1389 → BSIP1321 |
| BSIP1690 | trpC2 amyE::(PytmI′-lacZ cat) ΔytmJKLMN::aphA3 | BSIP1389 → BSIP1256 |
| BSIP1691 | trpC2 amyE::(PyhcL′-lacZ cat) ΔyhcL::spc | BSIP1534 → BSIP1321 |
| BSIP1692 | trpC2 amyE::(PytmI′-lacZ cat) ΔyhcL::spc | BSIP1534 → BSIP1256 |
| BSIP1693 | trpC2 amyE::(PyhcL′-lacZ cat) ΔytmJKLMN::aphA3 ΔyhcL::spc | BSIP1534 → BSIP1689 |
| BSIP1694 | trpC2 amyE::(PytmI′-lacZ cat) ΔytmJKLMN::aphA3 ΔyhcL::spc | BSIP1534 → BSIP1690 |
| BSIP1701 | trpC2 amyE::(PyhcL′-lacZ cat) ΔytlI::aphA3 | BSIP1214 → BSIP1321 |
| BFS1605 | trpC2 yhcL′::lacZ-erm ΔyhcL | S. Bron |
| BFS4376 | trpC2 yckK′::lacZ-erm ΔyckK | K. Yamane |
a
cat, pC194 chloramphenicol acetyltransferase gene; aphA3, Enterococcus faecalis kanamycin resistance gene; spc, Staphylococcus aureus spectinomycin resistance gene; erm, pMUTIN erythromycin resistance gene.
b
—, see Materials and Methods.