Table 1. Proteins unique or greatly expressed in MCPIP1- expressing MSCs when compared with Puro-treated MSCs.
| Proteins unique for MCPIP1-overexpressing MSCs | |||
|---|---|---|---|
| Accession number: | Protein name | Fold change | Selected function [Based on UniProtKB] |
| Q5D1E8 | Ribonuclease ZC3H12A | ND | Pro-inflammatory cytokines mRNA decay; angiogenesis |
| Q9CQB5 | CDGSH iron-sulfur domain-containing protein 2 | ND | Autophagy regulation |
| P62257 | Ubiquitin-conjugating enzyme E2 H | ND | Protein ubiquitination |
| Q6PFQ7 | Ras GTPase-activating protein 4 | ND | Ras protein signal transduction regulation |
| P70444 | BH3-interacting domain death agonist | ND | Caspases and apoptosis induction |
| Q8K3C3 | Protein LZIC | ND | Wnt/ β-catenin signaling |
| Q3UL36 | Arginine and glutamate-rich protein 1 | ND | ER-mediated transcription; required for cell growth |
| Quantitatively more in MCPIP1-overexpressing MSCs when compared with Puro | |||
| O35509 | Ras-related protein Rab-11B | 6.71 | Key regulators of intracellular membrane trafficking |
| P47226 | Testin | 5.43 | Negative regulation of cell proliferation |
| P70362 | Ubiquitin fusion degradation protein 1 homolog | 4.10 | Proteasome-mediated ubiquitin-dependent protein catabolic process |
| Q61074 | Protein phosphatase 1G | 3.63 | Cell cycle arrest |
| Q9ERN0 | Secretory carrier-associated membrane protein 2 | 3.56 | Recycling carrier to the cell surface |
| Q9Z2M7 | Phosphomannomutase 2 | 3.55 | Fructose and mannose metabolism |
| P48771 | Cytochrome c oxidase subunit 7A2, mitochondrial | 3.39 | Respiratory electron transport chain |
| Q8CI08 | SLAIN motif-containing protein 2 | 3.29 | Microtubule organization |
| P11730 | Calcium/calmodulin-dependent protein kinase type II subunit gamma | 3.02 | Sarcoplasmic reticulum Ca2+ transport in skeletal muscle |
| Q9JHR7 | Insulin-degrading enzyme | 2.98 | Degradation of insulin, glucagon and other polypeptides |
| Q8VCW4 | Protein unc-93 homolog B1 | 2.97 | Innate immune response |
| O08599 | Syntaxin-binding protein 1 | 2.87 | Unknown |
| P38060 | Hydroxymethylglutaryl-CoA lyase, mitochondrial | 2.74 | Unknown |
| Q11136 | Xaa-Pro dipeptidase | 2.73 | Collagen catabolic process |
| Q8K4M5 | COMM domain-containing protein 1 | 2.68 | Promotes ubiquitination of NF-kappa-B subunit RELA |
| O55012 | Phosphatidylinositol-binding clathrin assembly protein | 2.53 | Endocytosis |
| P49442 | Inositol polyphosphate 1-phosphatase | 2.36 | DNA synthesis inhibition |
| Q8BYK6 | YTH domain family protein 3 | 2.36 | mRNA splicing |
| O35682 | Myeloid-associated differentiation marker | 2.34 | Strongly up-regulated as multipotent progenitor cells differentiate towards myeloid cells |
| Q4KML4 | Costars family protein C6orf115 homolog | 2.32 | Unknown |
| Q61753 | D-3-phosphoglycerate dehydrogenase | 2.31 | L-serine biosynthetic process |
| Q8VC28 | Aldo-keto reductase family 1 member C13 | 2.31 | Xenobiotic metabolic process |
| P97807 | Fumarate hydratase, mitochondrial | 2.28 | Tricarboxylic acid cycle |
| Q8VBT0 | Thioredoxin-related transmembrane protein 1 | 2.26 | Cell redox homeostasis |
| Q3UX10 | Tubulin alpha chain-like 3 | 2.26 | Microtubule-based process |
| Q9JK38 | Glucosamine 6-phosphate N-acetyltransferase | 2.26 | Glucosamine metabolic process |
| Q91V33 | KH domain-containing, RNA-binding, signal transduction-associated protein 1 | 2.26 | G2/M transition of mitotic cell cycle; alternative splicing |
All listed proteins were identified in 2 samples based on two or more peptides identified for every protein; classified according to the fold change in expression based on global proteomic analysis. Selected functions of all listed proteins were assigned based on UniProtKB data base. ND- fold change in protein expression was not computed since the indicated proteins were not detected in Puro-treated cells.