FIG. 2.

Genomic comparison of five Brucella species. ORFs, deleted either partially or completely in five Brucella species relative to 16M, are shown. Putative deletions were selected if the average hybridization signal ratio was 4 SD below the mean. Each putative deletion was confirmed by PCR, followed by sequencing. ORFs in red had ratios lower than the average and were confirmed missing. ORFs in green and blue were confirmed missing by PCR and sequencing but did not have low ratios since the sequences were either present in multiple copies in the 16M genome (green) or were partially deleted (blue). ORFs that were unaltered relative to 16M are gray. An asterisk indicates ORFs in predicted islands for which the ratio was only 3 SD below the mean.