TABLE 2.
Oligonucleotide primers used to confirm the deletions identified by microarray hybridization
| ORFs in the deleted regions | Primer IDa | Sequence |
|---|---|---|
| Chromosome I | ||
| BMEI0284 | BMEI0283F | ATGGTCACGTCGAAATGCATCG |
| BMEI0285R | ACCAACTGGATGTTTCCGGCA | |
| BMEI0728 | BMEI0727F | GGTTCTGGGCTGCGATTCCA |
| BMEI0729R | CCGCAGCCTGTTTGGTCTCAA | |
| BMEI0801 | BMEI0800F | GATATGGACTGCTTCGTCGGCC |
| BMEI0802R | GTTGAAATGCCCAGCCGCTC | |
| BMEI0899-0907 | BMEI0898F | CGATCAATACGGTGGCCGATG |
| BMEI0908R | CGACAGGAATCAGTGCCGCA | |
| BMEI0899-900 | BMEI0898F | CGATCAATACGGTGGCCGATG |
| BMEI0901R | CCGCAATCGATCCCAAAAGG | |
| BMEI0926 | BMEI0925F | CTTTGGCCTTTCAAGCTCTCCATC |
| BMEI0927R | GCATCCGGTAAATGACCATCTGTTC | |
| BMEI0929 | BMEI0928F | GAGCGAGCGCCCGTTATCAT |
| BMEI0930R | TTTTCAGGGCAGGGGATTGG | |
| BMEI0993-1012 | BMEI0992F | CTATAGCAACTCCTGAACCGTTTCTGC |
| BMEI1014R | TGCCGTTCTTGGTACATCAGTGAAG | |
| BMEI1314 | BMEI1313F | GGCCAGTTCGCTCTTGCTCAT |
| BMEI1315R | GCTGGCTTTGCCCGAATAGG | |
| BMEI1435 | BMEI1434F | GCCAGCCACAGGATCAGGTGAT |
| BMEI1436R | GGATCCGTTCGTTTCGCTCG | |
| BMEI1657 | BMEI1656F | CAAAGCTCCAAGCATCTAATTTCAAGC |
| BMEI1658R | GCCATAGCTGTTGCTACGGTATGAATTA | |
| BMEI1661-1662 | BMEI1660F | AGCTTCCCGAACAAGAGCCTCC |
| BMEI1663R | TCCCTGCAATCCGCAAGGAC | |
| BMEI1674-1703 | BMEI1673F | AGAAGAATATCTCCCCTGCTTGCG |
| BMEI1704R | TCTCCATGATTTTGCCCGCC | |
| BMEI1747 | BMEI1746F | CACGACCGCAGCTTTCATCG |
| BMEI1748R | AGGACATGGCGGAATCGGAG | |
| BMEI1819 | BMEI1818F | GTACTCCCCCAGAGTATATAGTTTCGGG |
| BMEI1820R | GTCCAGAATGCGCAGGTGAAAG | |
| BMEI1873 | BMEI1872F | ACTGATATGCTGCCTTCGCCAG |
| BMEI1874R | AAAGATCTCATGCAGCGCATCG | |
| BMEI1896 | BMEI1895F | GGCTTGTCCGCATCCGAAAC |
| BMEI1897R | GCAAGGCCGGACACTTTTGAG | |
| BMEI1919-1923 | BMEI1919F | TCACGCGCATCCAGTTAGCC |
| BMEI1923R | GGCTGGCACTTCGCAATCCT | |
| Chromosome II | ||
| BMEII0129 | BMEII0128F | CCCTATCACGCCCATAACCGTC |
| BMEII0130R | AATTGGTCGTGCCGCGAAAG | |
| BMEII0185-BMEII0226 | BMEII0185F | CGCTATCTGGCCGAACGCTT |
| BMEII0226R | GAACGACAAGGGTGTTGGACCTG | |
| BMEII0291-0292 | BMEII0291F | AGGAAACCGAACGGATGCTGC |
| BMEII0293R | ATCGTCTGGTCTGGCGCCAT | |
| BMEII0405 | BMEII0404F | CCAACCCGATCGCGATGATC |
| BMEII0406R | CATATCGGCAACGGCTGGTTC | |
| BMEII0635-0636 | BMEII0634F | TGCTCCAGTTGCGCCAGATC |
| BMEII0637R | GTCTATCGTCACCATCGCACTGC | |
| BMEII0708 | BMEII0707F | CGGCGCTTCCATTTCATCAA |
| BMEII0709R | GATGGTTTCTCACTTCGGTACAATGG | |
| BMEII0717 | BMEII0715F | GAAGGCTCCCAAGCGTGCAA |
| BMEII0720R | GCCAACGGTTACATTGCAGC | |
| BMEII0710-0719 | BMEII0709F | GCTGATGCAGAATCGTTTGCG |
| BMEII0720R | GGTTCCATACGTGCTGCGTGA | |
| BMEII0811-0815 | BMEII0811F | GCAGCTTCACTGGACGATGTCG |
| BMEII0815R | TTTTCGACTTCCGCCTTGCG | |
| BMEII0826-0850 | BMEII0826F | GCGTGAAATGCCTCGGCATA |
| BMEII0850R | GCGGTTTCTTCCAGCCAGTTTC | |
| BMEII0875-0878 | BMEII0874F | TCAAGCGTGAGCATGTGCAAAC |
| BMEII0879R | ACAGAACGGCCAAAGCAGGATT | |
| BMEII0986-0988 | BMEII0985F | CACAAATAAACAGCTCGTC |
| BMEII0988R | GGTGGTGAATTTCTATTTC | |
| BMEII1016 | BMEII1015F | CCAGTGGAAAACTGGTTGTAAGCAA |
| BMEII1017R | TCTGGACAATTGGGAACATGACG | |
| BMEII1071 | BMEII1070F | CTGCAAAGTGCCGCCGTTAA |
| BMEII1072R | CGTCGATGGCGGAGGGAATA |
a
ID, identification designation.