Table 1. Ingenuity Pathway Analysis of disease and biomarkers pathways induced by TLR7 stimulation.
| Activation Z score a | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pathway | microglia | astrocyte | average | difference b | Top genes in each pathway: | |||||||||
| Highest Z scores pathways | Upregulated | Down-regulated | ||||||||||||
| microglia: | ||||||||||||||
| activation of blood cells | 6.04 | 5.62 | 5.83 | 0.42 | Il6 | Il1b | Il1a | Il12b | Ptgs2 | Cd300lf | Cd28 | Cd180 | Gmnn | Cx3cr1 |
| activation of leukocytes | 5.88 | 5.71 | 5.80 | 0.18 | Il6 | Il1b | Il1a | Il12b | Ptgs2 | Havcr2 | Cd300lf | CD28 | Cd180 | |
| activation of phagocytes | 5.33 | 4.71 | 5.02 | 0.63 | Il6 | Il1b | Il1a | Ptgs2 | Cxcl1 | Havcr2 | Cd300lf | |||
| recruitment of cells | 4.65 | 4.13 | 4.39 | 0.52 | Il6 | Il1b | Il1a | Cd69 | Ptgs2 | S1pr1 | Cd28 | Cxcr4 | Cx3cr1 | Gcnt1 |
| migration of cells | 4.60 | 3.92 | 4.26 | 0.68 | Il6 | Il1b | Il1a | Il12b | Cd69 | Hpgd | Ccnd1 | Cx3cr1 | Plau | Gcnt1 |
| astrocytes | ||||||||||||||
| activation of leukocytes | 5.88 | 5.71 | 5.80 | 0.18 | Il1a | Cxcl3 | Fpr1 | Il1b | Clec4e | Havcr2 | ||||
| activation of blood cells | 6.04 | 5.62 | 5.83 | 0.42 | Il1a | Cxcl3 | Fpr1 | Il1b | Clec4e | Cx3cr1 | Havcr2 | |||
| activation of phagocytes | 5.33 | 4.71 | 5.02 | 0.63 | Il1a | Cxcl3 | Fpr1 | Il1b | Clec4e | Havcr2 | ||||
| cell movement of monocytes | 4.16 | 4.16 | 4.16 | 0.00 | Cxcl3 | Ccl3l1 | Fpr1 | Il1b | Cxcl10 | — | ||||
| differentiation of cells | 3.68 | 4.14 | 3.91 | -0.47 | Il1a | Cd69 | Ccl3l1 | Il1b | Cxcl10 | Havcr2 | Gcnt1 | |||
| Greatest differences in Z scores | ||||||||||||||
| microglia | ||||||||||||||
| lymphatic node tumor | 2.41 | -0.29 | 1.06 | 2.71 | Il6 | Cd69 | Tnf | Cd40 | Gadd45b | Msh2 | Cxcr4 | Pola1 | Klhl6 | Ccnd1 |
| non-Hodgkin's disease | 2.41 | -0.29 | 1.06 | 2.71 | Il6 | Cd69 | Tnf | Gadd45b | Cxcl10 | Msh2 | Cxcr4 | Pola1 | Klhl6 | Ccnd1 |
| lymphomagenesis | 1.91 | 0.01 | 0.96 | 1.90 | Il6 | Cd69 | Nos2 | Tnf | Cd40 | Msh2 | Cxcr4 | Pola1 | Klhl6 | Ccnd1 |
| malignant lymphocytic neoplasm | 1.91 | 0.01 | 0.96 | 1.90 | Il6 | Il1b | Il1a | Il12b | Cd69 | Adrb2 | Ccnd1 | Cx3cr1 | Plau | Gcnt1 |
| astrocytes | ||||||||||||||
| quantity of IgG3 | 0.13 | 1.63 | 0.88 | -1.51 | Tnf | Tlr2 | Ptgs2 | Pik3ap1 | Rel | — | ||||
| cell cycle progression | 0.36 | 1.96 | 1.16 | -1.60 | Il1a | Il1b | Cxcl10 | Tnf | Il6 | Hpgd | ||||
| quantity of lymphatic system cells | 0.83 | 2.46 | 1.65 | -1.62 | Il1a | Tnf | Il6 | Nfkbia | Pik3ap1 | — | ||||
| quantity of interleukin | 0.50 | 1.65 | 1.07 | -1.14 | Il1b | Ccl2 | Tnf | Il6 | Tlr2 | — | ||||
a: Z score for each data set as calculated by IPA software. A score above 2.00 is significant. Bold scores for each group were used for ranking
b: difference in Z scores calculated by subtracting astrocyte Z score from microglia Z score