Table 3. mRNA expression following TLR9 ligand stimulation a .
| CpG-ODN (fold) b | Imiquimod/CpG-ODN d | ||||||
|---|---|---|---|---|---|---|---|
| Rank | Gene | micro | astro | Differencec | microglia | astrocytes | |
| Fig 2 (both) | 4 | Cd69 | 126.1 | 89.9 | 1.4 | 0.30 | 2.28 |
| 6 | AW112010 | 110.3 | 14.3 | 7.7 | 0.96 | 3.89 | |
| 8 | Ptgs2 (Cox 2) | 18.8 | 2.2 | 8.7 | 1.62 | 2.03 | |
| 9 | Irg1 | 220.8 | 27.7 | 8.0 | 0.88 | 2.28 | |
| 13 | Fpr1 | 98.5 | 22.0 | 4.5 | 0.80 | 1.83 | |
| 14 | Gbp5 | 268.8 | 10.8 | 24.9 | 1.65 | 2.61 | |
| 22 | Tnfrsf1b | 3.9 | 5.2 | 0.7 | 1.22 | 1.99 | |
| 23 | Irak3 | 4.9 | 7.2 | 0.7 | 1.42 | 2.22 | |
| 25 | Malt1 | 7.1 | 3.0 | 2.3 | 1.61 | 2.79 | |
| 27 | Gpr84 | 27.0 | 8.1 | 3.3 | 0.79 | 1.84 | |
| 29 | Nfkbiz | 39.6 | 9.0 | 4.4 | 0.71 | 1.58 | |
| 32 | Tnfaip2 | 5.9 | 8.9 | 0.7 | 0.50 | 1.53 | |
| 35 | Casp4 | 7.3 | 3.0 | 2.4 | 1.01 | 1.60 | |
| 36 | Birc3 | 5.8 | 4.4 | 1.3 | 0.76 | 1.47 | |
| Fig 3 (microglia) | 1 | Marco | 620.0 | 26.3 | 23.6 | 0.91 | 2.62 |
| 4 | Saa3 | 692.9 | 45.8 | 15.1 | 4.44 | 4.08 | |
| 6 | Gbp2 | 25.5 | 3.8 | 6.7 | 1.24 | 2.13 | |
| 9 | Traf1 | 74.8 | 2.7 | 27.4 | 2.12 | 2.50 | |
| 30 | Nlrp3 | 15.4 | 10.0 | 1.6 | 0.48 | 1.99 | |
| 31 | Ifit1 | 20.3 | 2.5 | 8.1 | 0.25 | 2.23 | |
| 50 | Ifi202b | 6.1 | 1.2 | 5.0 | 0.69 | 1.48 | |
| 69 | Zc3h12c | 3.1 | 3.8 | 0.8 | 1.65 | 1.78 | |
| Fig 4(astrocytes) | 7 | Elovl7 | 1.9 | 2.9 | 0.7 | 0.97 | 1.52 |
| 8 | Cxcl11 | 95.4 | 10.6 | 9.0 | 0.65 | 1.90 | |
| 4 | BC023105 | 78.0 | 3.4 | 23.1 | 0.41 | 1.95 | |
| 3 | Rapgef5 | 1.4 | 2.6 | 0.5 | 0.62 | 1.17 | |
a: bold cells: increase >2 fold, italicize cells: decrease > 2 fold b: fold change in gene expression from CpG-ODN stimulated cells compared to mock as determined by real-time PCRd: difference between microglia and astrocytes in gene expressione: difference in fold expression induced by imiquimod vs CpG-ODN stimulation as determine by real-time PCR