FIG. 6.

Catabolic cassette for the catabolism of Tyr. (A) Schematic representation of the construction and expression of a Tyr catabolic cassette. The primer pairs used for PCR amplification are shown in Table 2. Genes are indicated by arrows. The Plac and Phmg promoters are shown. Ap^r and Km^r, genes that confer ampicillin and kanamycin resistance, respectively. The pBBR1 and pUC origins of replication (oripBBR1 and oripUC) are indicated. oriTRP4, RP4-mediated mobilization (Mob⁺) functions. (B) Growth of recombinant strain E. coli(pU-HHP) (•) and control strain E. coli(pUC18) (▴) in MM supplemented with 0.05% Casamino Acids and 5 mM Tyr as the sole carbon and energy source.