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Effect of global regulators on the production of acyl-HSLs by S. plymuthica IC1270. Acyl-HSLs were detected by TLC on RP-18 plates with A. tumefaciens NTL4/pZLR4 overlay. Lanes: 1, mixture of HHHL (10 μg ml−1, 8 μl) and HOHL (1 μg ml−1, 5 μl) standards; 2, 3, 4, and 5, ethyl acetate extracts of strains IC1270, IC1270#20, IC1270#93, and IC1270#823, respectively, 20 μl of each; 6, ethyl acetate extract of strain 2-79, 5 μl.
