TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Agrobacterium tumefaciens NTL4/pZLR4 | Gmr Cbr; β-galactosidase induction-based acyl-HSL bioreporters | 41 |
| Chromobacterium violaceum CV026 | Violacein production-based acyl-HSL bioreporter | 26 |
| Escherichia coli | ||
| DH5α | F−endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 φ80dlacZΔM15 λ− | 3 |
| S17-1(λ-pir) | thi pro hsdR hsdM recA rpsL RP4-2 (Tcr::Mu) (Kmr::Tn7) | 42 |
| Pseudomonas fluorescens 2-79 | Phz+ | 43 |
| Serratia plymuthica (previously Enterobacter agglomerans) | ||
| IC1270 | Rifr, isolated from rhizosphere of grape in Samarkand region, Republic of Uzbekistan | 8 |
| IC1270#20 | grrA (=gacA) Rifr Gmr gene replacement mutant of strain IC1270 | This study |
| IC1270#93 | grrS (=gacS), Rifr Gmr gene replacement mutant of strain IC1270 | This study |
| IC1270#823 | rpoS Rifr Gmr gene replacement mutant of strain IC1270 | This study |
| Plasmids | ||
| pSB401 | Tcr, lux-based acyl-HSL bioreporter | 47 |
| pSB536 | Ampr, lux-based acyl-HSL bioreporter | 47 |
| pSB1075 | Ampr, lux-based acyl-HSL bioreporter | 47 |
| pGEM-T Easy | Ampr | Promega Corp. |
| p34S-Gm | Source of Gmr cassette | 11 |
| pEX18Tc gene replacement vector | TcroriT+sacB | 21 |
a
Ampr, ampicillin resistance; Gmr, gentamicin resistance; Tcr, tetracycline resistance; Rifr, rifampicin resistance; Cbr, carbenicillin resistance; sacB, sucrose-inducible gene of Bacillus subtilis, the expression of which is lethal in the presence of 5% sucrose; Phz+, production of phenazine-1-carboxylic acid.