FIG. 2.
Effect of SscB on the stability of SseF. The secretion-deficient strains ZP18 (ΔssaV) and ZP41 (ΔssaV sscB::aphT), harboring pZP281(encoding SseF-M45) or pZP430 (encoding SifB-M45), were grown in MgM7, and protein expression was induced with 1 mM arabinose. Gentamicin (100 μg ml−1) was then added to inhibit protein synthesis. (A) The levels of SseF-M45 and SifB-M45 were examined at different times after gentamicin treatment by Western blotting with anti-M45 antibody. (B) The protein amounts were quantified by densitometry scanning. Experiments were repeated three times.