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Effect of SscB on the secretion of SseF. Secretion of SseF-M45 (pZP281), SifB-M45 (pZP430), and SseJ-M45 (pZP492) were detected in either wild type (SL1344) or an sscB mutant strain (ZP22). Bacteria were grown in MgM5, and the culture supernatants were collected after centrifugation. Secreted proteins were precipitated and analyzed as described in Materials and Methods.
