FIG. 5.

SseF interacts with SscB in a coprecipitation assay. Serovar Typhimurium mutant strain ZP36 (ΔssaV ΔsseF sscB::aphT) containing plasmids expressing SseF-M45 and His-SscB (pZP434), SseF-M45 and SscB (pZP284), or SseF-M45 and His-ADF (pZP445) was grown in MgM7, and protein expression was induced with 1 mM arabinose. His-tagged proteins were precipitated from lysates by Ni-NTA agarose beads. Samples were taken before the addition of the beads (Pre) and after bead binding (Post). Coprecipitated proteins (Pull-down), Pre, and Post samples were all examined by Western blotting with the anti-M45 antibody.