FIG. 6.

The sscB mutation induced discontinuous Sifs. HeLa cells were infected with wild-type (SL1344), the sseF mutant (ZP19), or the sscB mutant (ZP22) strains. Sifs and vacuolar membranes were labeled with mouse anti-LAMP-2 H4B4 antibody and secondary Alexa Fluor 488 (green). Bacteria were visualized by staining with rabbit anti-O antigen and secondary antibody-Texas red (red). Arrows indicates Sifs, and arrowheads indicate the “pseudo-Sifs.”