FIG. 7.

SscB is required for efficient intracellular survival of serovar Typhimurium in macrophages. RAW264.7 macrophages were infected with wild-type serovar Typhimurium and various mutant strains at an MOI of 10. Extracellular bacteria were removed by washing and gentamicin treatment. At 2 and 24 h after bacterial invasion, cells were lysed and the number of intracellular bacteria was determined by plating on LB medium-streptomycin plates. The data shown were obtained from three independent experiments, each performed in duplicate.