TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| S. enterica serovar Typhimurium | ||
| SL1344 | Wild-type serovar Typhimurium; Strr | 23 |
| ZP18 | ΔssaV | This study |
| ZP19 | ΔsseF | This study |
| ZP22 | sscB::aphT | This study |
| ZP31 | ΔsseF::lacZ | This study |
| ZP33 | ΔsseF::lacZ sscB::aphT | This study |
| ZP39 | ΔssaV::lacZ | This study |
| ZP40 | ΔssaV::lacZ sscB::aphT | This study |
| ZP36 | ΔssaV ΔsseF sscB::aphT | This study |
| ZP41 | ΔssaV sscB::aphT | This study |
| E. coli | ||
| DH5αMCR | F−mcrA Δ(mrr-hsdRMS-mcrBC) | Gibco-BRL |
| SM10λpir | thi thr leu tonA Δacy supE recA::RP4-2-Tc::Mu(Kmr)λpir F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 | 31 |
| Top10 | deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pSB890 | R6K-derived ori; Tcr | 24 |
| pWSK29 | pSC101 ori; Apr | 48 |
| pZP226 | ΔssaV | This study |
| pZP227 | ΔsseF | This study |
| pZP259 | sscB::aphT | This study |
| pSB1040 | Promoterless lacZ gene | 34 |
| pZP369 | ΔsseF::lacZ | This study |
| pZP428 | ΔssaV::lacZ | This study |
| pZP281 | Arabinose-inducible sseF-M45 | This study |
| pZP284 | Arabinose-inducible sscBsseF-M45 | This study |
| pZP430 | Arabinose-inducible sifB-M45 | This study |
| pZP434 | Arabinose-inducible bicistronic sseF-M45sscB-his | This study |
| pZP445 | Arabinose-inducible bicistronic sseF-M45 ADF-his | This study |
| pZP492 | Arabinose-inducible sseJ-M45 | This study |
| pZP545 | ProsseA in pWSK29 | This study |
| pZP546 | sseF-M45 under ProsseA | This study |
| pZP549 | sscBsseF-M45 under ProsseA | This study |
| pZP548 | sscB-M45 under ProsseA | This study |
a
Strr, streptomycin resistance; Kmr, karamycin resistance; Tcr, tetracycline resistance; Apr, ampicillin resistance.