TABLE 1.

Bacterial strains, plasmids, and oligonucleotides

Strains, plasmids, and oligonucleotides	Relevant characteristicsa	Source or reference
Strains
E. coli
JM103	hsdR4 Δ(lac-pro) F′ traD36 proAB laclqZΔM15	5
JM109	hsdR17 Δ(lac-pro) F′ traD36 proAB laclqZΔM15	5
XL1-Blue	supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F′ [proAB+laclqlacZΔM15 Tn10(Tetr)]	5
M15(pREP4)	thi lac ara+gal mtl F′ recA+uvr+lon+; pREP4 plasmid Kanr	Qiagen
R. sphaeroides WS8	Wild type; spontaneous Nalr	22
Plasmids
pQE30	Expression vector; Apr, N-terminal His6 tag	Qiagen
pQE60	Expression vector; Apr, C-terminal His6 tag	Qiagen
pRSM	fliM cloned into the NcoI/BglII sites of pQE60	This work
pRSMΔ13	fliMΔ13 cloned into the NcoI/BglII sites of pQE60	This work
pRSMΔ20	fliMΔ20 cloned into the NcoI/BglII sites of pQE60	This work
pRSY1	cheY1 cloned into the NcoI/BamHI sites of pQE60	This work
pRSY2	cheY2 cloned into the SacI/PstI sites of pQE30	This work
pRSY3	cheY3 cloned into the BamHI/PstI sites of pQE30	This work
pRSY4	cheY4 cloned into the NcoI/BamHI sites of pQE60	This work
pRSY5	cheY5 cloned into the BamHI/PstI sites of pQE30	This work
pRSY6	cheY6 cloned into the BamHI/PstI sites of pQE30	This work
Oligonucleotidesb
cheY1 fw	5′-CATGCCATGGCGCCGCTGACCGTTCTTGCC-3′	This work
cheY1 rev	5′-CTCACGGACTGCTCGGATGAAGCC-3′	This work
cheY2 fw	5′-CCGAGCTCGTGGTCGATGACATGTCGACCAGC-3′	This work
cheY2 rev	5′-AAAACTGCAGTCATAGAGCGCCTACGACAGC-3′	This work
cheY3 fw	5′-CGCGGATCCAGCAGGACGGTTCTCGCCGTG-3′	This work
cheY3 rev	5′-AAAACTGCAGTCATCCGAGCACCTTCTTGAT-3′	This work
cheY4 fw	5′-CATGCCATGGCGACGAAAACCGTCCTCGCA-3′	This work
cheY4 rev	5′-CGCGGATCCGGCCAAGAAGCTTCTTCATCAC-3′	This work
cheY5 fw	5′-CGCGGATCCAGCAAGACGATCCTCGCGGTGGAT-3′	This work
cheY5 rev	5′-AAAACTGCAGTCAGGCGCCGGCCACCTTCTTGAC-3′	This work
cheY6 fw	5′-GCGGGATCCCCCTACAATGTCATGATC-3′	This work
cheY6 rev	5′-AAAACTGCAGTCAGGCGGCCATCAGCGT-3′	This work

^aAp^r, ampicillin resistance; Kan^r, kanamycin resistance; Nal^r, nalidixic acid resistance.

^bfw, forward; rev, reverse.