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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Nature. 2015 Apr 27;521(7551):232–236. doi: 10.1038/nature14443

Figure 2. SHARP, LBR, and SAF-A are required for Xist-mediated gene silencing.

Figure 2

(a) Screen for Xist-mediated gene silencing for knockdown of control (top), non-silencing proteins (middle), or silencing proteins (bottom). (b) Gpc4 mRNA levels after induction of Xist (+dox) normalized to Gpc4 levels before Xist induction (−dox). Error bars: standard error of the mean across 50 cells from one experiment. siCtrl: scrambled siRNA control. (c) Images of individual cells for two X-linked mRNAs, Gpc4 (green) and Atrx (red), and DAPI (blue) after treatment with different siRNAs (rows). The number of identified mRNAs is shown. Scale bars, 5 micrometers.