The differences of mRNA expression of p35, EBI3, gp130, IL-12Rβ2 and forkhead box protein 3 (FoxP3) of thymic cells between interleukin (IL)-35 and phosphate-buffered saline (PBS) treatment. Total RNA extracted from thymic cells from Murphy Roths Large (MRL)/lpr and BALB/c mice was reverse-transcribed and analysed by quantitative polymerase chain reaction (qPCR). Results were presented as bar charts showing the arithmetic mean plus standard error of the mean (s.e.m.). Differences among each group of mice in the same treatment were compared by Kruskal–Wallis analysis of variance (anova), followed by Dunn’s post-test. †P < 0·05, BALB/c control mice versus PBS-treated MRL/lpr mice; ‡P < 0·05, BALB/c control mice versus IL-35-treated MRL/lpr mice (n = 5 in each group).