Expression of gp130 and IL-12Rβ2 on splenic CD4+ T helper (Th) cell surface in Murphy Roths Large (MRL)/lpr and BALB/c mice with interleukin (IL)-35 or phosphate-buffered saline (PBS) treatment. Isolated splenic cells were stained with immunofluorescent antibodies and analysed by flow cytometry. Cell surface expression of gp130 and IL-12Rβ2 on CD4+ Th cells was presented as bar charts with the arithmetic mean plus standard error of the mean (s.e.m.). *P < 0·05, **P < 0·01, ***P < 0·001, IL-35 versus phosphate-buffered saline (PBS) treatment; †P < 0·05, ††P < 0·01, BALB/c control mice versus PBS-treated MRL/lpr mice; ‡P < 0·05, BALB/c control mice versus IL-35-treated MRL/lpr mice (n = 5 in each group).