Plasma concentrations of cytokines in Murphy Roths Large (MRL)/lpr and BALB/c mice with interleukin (IL)-35 or phosphate-buffered saline (PBS) treatment. Bar charts are shown with the arithmetic mean values of the plasma levels of (a) interferon (IFN)-γ, (b) IL-12p70, (c) IL-21, (d) IL-2, (e) IL-27, (f) IL-10, (g) tumour necrosis factor (TNF)-α, (h) IL-6 and (i) IL-17A.*P < 0·05, **P < 0·01, IL-35 versus PBS treatment; †P < 0·05, ††P < 0·01, †††P < 0·001, BALB/c control mice versus PBS-treated MRL/lpr mice; ‡P < 0·05, BALB/c control mice versus IL-35-treated MRL/lpr mice (n = 5 in each group).