Figure 6.

Effect of gentamycin on expression of RYRs in portal vein muscle. (A) Relative mRNA expression levels of the three RYR sub-types were revealed by RT-PCR (35 cycles) and compared with GAPDH expression in control (open bars), mdx (filled bars) and gentamycin-treated mdx mice (hatched bars). (B) RYR sub-types were revealed in proteins extracted (100 μg) from control, mdx and gentamycin-treated mdx mice. Results from four control (open bar), mdx (filled bar) and gentamycin-treated mdx mice (hatched bars) preparations were normalized to the loading control. Data are means ± S.E. with the number of experiments in parentheses. ⋆, values significantly different from those obtained in control mice (P < 0.05).