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. 2009 Mar 6;13(9b):3218–3225. doi: 10.1111/j.1582-4934.2009.00732.x

Figure 2.

Figure 2

Cav1 regulation of CT-b endocytosis requires an intact scaffolding domain but not phosphorylation on tyrosine 14. (A) Mgat5−/–ESC cells were treated with mβCD for 30 min. or infected with adenoviruses encoding for clathrin-hub, dynamin-wt, dynamin K44A or Cav1 and then incubated with CT-b-FITC (green) for 30 min. at 37°C. Cells were then fixed and labelled with GM130 (red). Co-localization of CT-b and Golgi was quantified for the indicated experimental conditions. (B) Mgat5−/–ESC cells were transfected with Cav1-wt or Cav1 Y14F and F92A/V94A mutants and processed for endocytosis. Cells were then fixed and labelled with anti-GM130 and anti-myc antibodies to identify transfected cells and CT-b-FITC/Golgi co-localization was quantified. *P < 0.01 relative to control cells.