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. 2009 Mar 6;13(9b):3218–3225. doi: 10.1111/j.1582-4934.2009.00732.x

Figure 4.

Figure 4

Cav1 and dynamin regulate raft-dependent CT-b endocytosis at the cell surface. (A) Mgat5−/–ESC cells were not treated (Control), treated with mβCD or infected with adenoviruses encoding for clathrin-hub, dynamin K44A and Cav1 and incubated with CT-b-FITC at 37°C for 30 min. and cell surface CT-b removed by acid washing. Alternatively, cells were not incubated with CT-b (Untreated), not acid-washed (wo A.W.) or incubated with CT-b-FITC at 4°C. Prior to flow cytometry, cells were incubated with propidium iodide (PI) to identify dead cells. Representative flow cytometry profiles are shown for PI and CT-b-FITC intensities under the various conditions, as indicated. (B) Percent positive cells was determined following incubation with CT-b-FITC (left) or Tf-FITC (right) for the conditions indicated earlier. Cells were acid washed in order to remove surface-bound CT-b-FITC or Tf-FITC (black bars) except where indicated (wo A.W., white bars).