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. 2009 Feb 27;13(9b):3268–3282. doi: 10.1111/j.1582-4934.2009.00726.x

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© 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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NDP-MSH binding assay of the WT and mutant hMC4Rs transiently expressed in HEK293T cells. HEK293T cells were transiently transfected with the indicated hMC4R constructs and binding assays were performed as described in ‘Materials and methods’. Different concentrations of unlabeled NDP-MSH (A) or AgRP(86–132) (B) were used to displace the binding of [¹²⁵I]-NDP-MSH to hMC4Rs on intact cells. Results are expressed as percentage of the maximal binding for WT hMC4R from duplicate determinations within one experiment. Shown are mean ± S.E.M. All experiments were performed at least three times.

NDP-MSH binding assay of the WT and mutant hMC4Rs transiently expressed in HEK293T cells. HEK293T cells were transiently transfected with the indicated hMC4R constructs and binding assays were performed as described in ‘Materials and methods’. Different concentrations of unlabeled NDP-MSH (A) or AgRP(86–132) (B) were used to displace the binding of [¹²⁵I]-NDP-MSH to hMC4Rs on intact cells. Results are expressed as percentage of the maximal binding for WT hMC4R from duplicate determinations within one experiment. Shown are mean ± S.E.M. All experiments were performed at least three times.