Table 1.
Primary and secondary antisera used for immunocytochemistry
| Primary antisera | |||
|---|---|---|---|
| Antigen | Host | Dilution | Source |
| CGRP | Rabbit | 1/5000 | Sigma, St. Louis, MO, USA (C8198) |
| GFAP | Rabbit | 1/500 | DAKO, Glostrup, Denmark (Z0334) |
| mMCP-1 | Rat | 1/200 | Prof. Dr. HRP Miller, Edinburgh, UK |
| nNOS | Rabbit | 1/1000 | EuroDiagnostica, Malmö, Sweden (B220–1) |
| PECAM-1 | Goat | 1/500 | Santa Cruz Biotechnology, Santa Cruz, CA, USA (sc-1506) |
| PGP | Rabbit | 1/1000 | Biogenesis, Poole, UK (7863--2004) |
| S100 | Rabbit | 1/5000 | DAKO (Z0311) |
| SOM | Rat | 1/100 | Biogenesis (8330--0009) |
| SSTR1 | Rabbit | 1/500 | Biotrend, Köln, Germany (SS-840) |
| SSTR2A | Rabbit | 1/2000 | Biotrend (SS-800) |
| SSTR3 | Rabbit | 1/500 | Biotrend (SS-850) |
| SSTR4 | Rabbit | 1/500 | Sigma (S0945) |
| SSTR5 | Rabbit | 1/200 | Abcam, Cambridge, UK (ab13121) |
| substance P | Rat | 1/100 | Biogenesis (8450–0505) |
| Secondary and tertiary antisera | Dilution | Source |
|---|---|---|
| Cy™-3-conjugated donkey anti-rabbit IgG | 1/500 | Jackson Immunoresearch, West Grove, PA, USA |
| Cy™-3-conjugated donkey anti-rat IgG | 1/500 | Jackson Immunoresearch |
| Cy™-5-conjugated Fab fragments of goat anti-rabbit IgG | 1/400 | Jackson Immunoresearch |
| FITC-conjugated goat anti-rat IgG | 1/100 | Jackson Immunoresearch |
| FITC-conjugated donkey anti-rabbit IgG | 1/100 | Jackson Immunoresearch |
| Biotinylated Fab fragments of goat anti-rabbit IgG | 1/2000 | Rockland, Gilbertsville, PA, USA |
| Biotin-conjugated tyramide signal amplification kit | PerkinElmer Life Sciences, Boston, MA, USA |
PGP = protein gene product 9.5, nNOS = neuronal nitric oxide synthase, GFAP = glial fibrillary acidic protein, mMCP-1 = mouse mast cell protease-1, PECAM-1 = platelet/endothelial cell adhesion molecule.