TABLE 2.
Strains used in this study
| Strain | Relevant characteristics | Source, reference, and/or constructiona |
|---|---|---|
| 168 | trpC2 | Laboratory collection |
| SU347 | divIC355 | 13 |
| MB76 | trpC2 divIBm12 | Laboratory stock, allele tms-12 (4) |
| 2083 | trpC2 ponA::pSG1492 (cat Pxyl-gfp-ponA1-394) | 19 |
| 3122 | trpC2 pbpB::pSG5061 (cat Pxyl-gfp-pbpB1-825) | 19 |
| 3295 | chr::(Pspac-pbpB neo) | 11 |
| 3511 | trpC2 ΔponA::spc | PS2062 (17) → 168 (Spcr)b |
| 3526 | trpC2 divIBm12 ponA::pSG1492 (cat Pxyl-gfp-ponA1-394) | 2083 → MB76 (Cmr) |
| 3536 | divIC355 ponA::pSG1492 (cat Pxyl-gfp-ponA1-394) | 2083 → SU347 (Cmr) |
| 3542 | trpC2 ponA::pSG1492 (cat Pxyl-gfp-ponA1-394) chr::(Pspac-pbpB neo) | 2083 → 3295 (Cmr) |
| 3907 | trpC2 ΔponA::spc chr::(Pspac-pbpB neo) | 3295 → 3511 (Kanr) |
| 3908 | trpC2 ΔponA::spc pbpB::pSG5061 (cat Pxyl-gfp-pbpB1-825) | 3122 → 3511 (Kanr) |
a
Spcr, spectinomycin resistance; Cmr, chloramphenicol resistance; Kanr, kanamycin resistance.
b
Strain 168 transformed to spectinomycin resistance with PS2062 chromosomal DNA.