Figure 4.

Role of the ribosomal–translocon complex. (A) SH-SY5Y cells were transiently cotransfected with the cDNAs coding for ER-Aeq and PS2-T122R (or with the void vector as control). Upon ER-Aeq reconstitution, cells were treated for 10 min. with puromycin (0.2 mM) at 37°C and continuously maintained in the presence of the drug. ER Ca²⁺ levels were measured as described in Fig. 1A. (B) SH-SY5Y cells were transfected as described in (A). Anisomycin (0.2 mM) was present during ER-Aeq reconstitution and throughout the experiments. Bars represent the average [Ca²⁺]_ER (μM) (mean ± S.E.M.).