Table 1.

Biopsies from tumour stroma formation in colorectal adenocarcinoma, pannus formation in rheumatoid arthritis and cutaneous healing wounds

Percentage of smooth muscle α-actin-positive pixels that are also positive for	Colorectal adenocarcinoma	Rehumatoid arthritis	Wound healing
HMW-MAA	28 ± 11	49 ± 23	47 ± 19
PDGF β-receptor	33 ± 14	47 ± 16	46 ± 22
Integrin α1 subunit	80 ± 11	72 ± 9	86 ± 14
Integrin α5 subunit	80 ± 11	72 ± 14	86 ± 15
PAL-E	14 ± 8	22 ± 11	20 ± 10

Biopsies were stained by a double immunofluorescence technique with various combinations of mAbs. Co-localization between two different markers is depicted as “percentage of pixels that are positive for both marker X and Y”. Stained sections were analysed by computer imaging processing, as detailed in Materials and methods. The recorded percentage values represent the spatial distribution of two mAb markers in relation to each other, measured in percentage of pixels that co-localize (1 pixel equals 0.9 μm × 0.9 μm) [26, 35].